Research Assistant I - Gene Expression Laboratory, Dr. Jesse Dixon
Under direct supervision, the Research Assistant I position is part of a multidisciplinary team studying epigenetics, chromatin structure, and gene expression. Responsibilities include, but are not limited to, performing techniques related to cell and molecular biology and biochemistry. Tasks include the maintenance of tissue, DNA, and other experimental samples, as well as taking part in data analysis and experimental planning. Other duties include assisting with general laboratory maintenance, including equipment maintenance, ordering supplies, etc.
The Dixon Lab seeks to interrogate the role of 3D genome architecture and chromatin regulation in both normal cellular biology and in cancer initiation and progression. Harnessing the power of genomic tools and advances in genome engineering, we are investigating the relationship between TADs (topologically associating domains) and subsequent gene expression. We utilize and develop cutting-edge genomics methods to understand the principles of how our genomes are organized in 3D space, and how genome organization affects gene regulation in normal and diseased cells. Our lab also uses CRISPR/Cas9 genome engineering approaches to investigate the effects of genetic variation on genome organization. By integrating genomic approaches, we aim to uncover insights into the structural and epigenetic landscape of cancer progression. Successful candidates will be eager to acquire experimental knowledge to leverage next-generation sequencing techniques and answer fundamental biological questions. Applicants will work in a team setting within the lab but will also have the opportunity to take on and lead independent projects over time.
Molecular and Cell Biology and Biochemistry Techniques (80%)
- Preparation of libraries for high-throughput/next-generation DNA sequencing.
- Performing experiments including ChIP-seq and RNA-seq.
- DNA Cloning, PCR, Isolation, and purification of RNA, Real-time PCR, Maxi and Mini preps of DNA, Restriction digestion of DNA, and Transformation of E. coli.
- Protein isolation from mouse tissue, Protein determination, Immunoprecipitation, Western blotting.
- Mammalian cell culture, transfections, infections, lysing, thawing, and freezing down cells.
- Maxi preps.
- Gel Electrophoresis.
- Recombinant DNA techniques.
Laboratory Support (20%)
- Perform general laboratory organization, cleaning, and maintenance.
- Stock and order supplies as needed.
- No professional experience is required with a relevant degree
- Demonstrated experience and application of molecular biology knowledge preferably in biochemistry and cell culture.
- Demonstrated experience with general lab maintenance, including cleaning, stocking supply inventory, and ensuring the maintenance of a sterile environment.
- Bachelor's degree in biological sciences or related field.
The salary range for this position is $18.03 - $23.92 hourly.
SKILLS AND ABILITIES
- Theoretical and hands-on knowledge of biology techniques.
- Demonstrated knowledge and experience in researching information and analyzing data to arrive at valid conclusions, recommendations, and plans of action.
- Ability to learn and carry out new experimental procedures with accuracy and efficiency. Demonstrated ability to follow verbal and/or written protocols, including follow-up on previously begun procedures.
- Demonstrated ability to record data accurately and enter it into the appropriate medium.
- Skill in the operation and maintenance of various lab equipment.
- Demonstrated knowledge and experience with both PC and MAC operating systems, standard business office equipment, and commonly used software and tools, such as Microsoft Office, electronic mail, and the internet.
- Proficiency with common office software applications as well as software applications used for research, recording, and analysis.
- Highly organized, effective at multitasking, adept at juggling changing priorities
- Proven ability to work both independently and also as part of a collaborative team.
- Excellent communication skills, both oral and written.
- Strong interpersonal skills, including tact, diplomacy, and flexibility.
- Demonstrated ability to interact effectively with diverse groups. Demonstrated skill in establishing and maintaining effective working relationships with personnel at all levels.
SPECIAL CONDITIONS OF EMPLOYMENT
- Must be willing to work in an animal-related research environment.
- Must be willing to work an adjusted schedule to support research needs.
- Successful completion of the Institute’s background investigation.
- Must be willing to sign a confidentiality agreement.
PHYSICAL REQUIREMENTS/MENTAL ACTIVITIES/ENVIRONMENTAL CONDITIONS
The incumbent in this position will be constantly seeing and frequently adjusting focus, grasping, hearing, sitting, standing, analyzing, communicating, reasoning, and working indoors.
Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities
The contractor will not discharge or in any other manner discriminate against employees or applicants because they have inquired about, discussed, or disclosed their own pay or the pay of another employee or applicant. However, employees who have access to the compensation information of other employees or applicants as a part of their essential job functions cannot disclose the pay of other employees or applicants to individuals who do not otherwise have access to compensation information, unless the disclosure is (a) in response to a formal complaint or charge, (b) in furtherance of an investigation, proceeding, hearing, or action, including an investigation conducted by the employer, or (c) consistent with the contractor’s legal duty to furnish information. 41 CFR 60-1.35(c)